What it does
3D PSF measurement
Characterize the point spread function of your microscope from sub-resolution fluorescent bead images — widefield, confocal, lightsheet, and more.
AI bead detection
Automated bead finding using AI-powered detection — no manual selection required. Works reliably even in crowded or noisy fields.
Reproducible output
Exports measured PSFs in standard formats compatible with Arnas Scope, ImageJ/Fiji, and other deconvolution tools. Full parameter logs for reproducibility.
Free and open source
Source code, documentation, and example datasets on GitHub.